It’s Time To “Buck” Up And Implement Bacteria Biofuel Solution

As syngas fermentation leads to lower ethanol concentrations than corn fermentations, the energy and cost to separate the ethanol from water is proportionally higher. To reduce this differential, Coskata has exclusively licensed membrane separation technology to reduce the energy requirements by over 50%. The vapor permeation process is amenable to separating ethanol from biofermentation broth because of the very low solids content of the broth relative to other fermentation processes. Image Credit: Coskata

It’s Time To “Buck” Up And Implement Bacteria Biofuel Solution

There was a time one could buy fuel for ones car or truck for a “Buck” a gallon … and it is a past we can embrace right now … TODAY!

Well, at least General Motors seems to think so with its investment in Biofuel processing startup Coskata.

The key to the conversion approach Coskata has perfected uses bacteria to break down the broad array of organic waste (switch grasses, twigs, corn husks, leaves, landscape waste, and other non-food sources of organic material) and make Ethanol for a fuel mix or replacement.

After the carbon-hydrogen bonds in the feedstock are "cracked" using gasification and converted into syngas, bacterial fermentation (biofermentation) of the syngas into ethanol occurs using proprietary Coskata microorganisms. Image Credit: Coskata

The real kicker is that this process not only protects our current food paradigm built upon corn for feed and food, the process uses far less petroleum fuel (about 13% as opposed to 77% - or 17 times more efficient) and water while greatly increasing the productive output per bale of feedstock in order to create a gallon of this cleaner burning substance.

Design engineer Mike Sura adjusts settings on Coskata's 150L bioreactor to make ethanol. Image Credit: Tyler Mallory/General Motors

This excerpted from WIRED -

Startup Says It Can Make Ethanol for $1 a Gallon, and Without Corn
By Chuck Squatriglia - 01.24.08 1:00 PM

A biofuel startup in Illinois can make ethanol from just about anything organic for less than $1 per gallon, and it wouldn't interfere with food supplies, company officials said.

Coskata, which is backed by General Motors and other investors, uses bacteria to convert almost any organic material, from corn husks (but not the corn itself) to municipal trash, into ethanol.

"It's not five years away, it's not 10 years away. It's affordable, and it's now," said Wes Bolsen, the company's vice president of business development.

The discovery underscores the rapid innovation under way in the race to make cellulosic ethanol cheaply. With the Energy Independence and Security Act of 2007 requiring an almost five-fold increase in ethanol production to 36 billion gallons annually by 2022, scientists are working quickly to reach that breakthrough.
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Besides cutting production costs to fire sale prices, the process avoids some key drawbacks of making ethanol from corn, company officials said. It wouldn't impact the food supply, and its net energy balance is high because the technique works almost anywhere using almost anything with great efficiency. The end result will be E85 sold at the pump for about a dollar cheaper per gallon than gasoline, according to the company.

Coskata won't have a pilot plant running until this time next year, and it will produce just 40,000 gallons a year. Still, several experts said Coskata shows enough promise to leave them cautiously optimistic.
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Coskata uses existing gasification technology to convert almost any organic material into synthesis gas, which is a mix of carbon monoxide and hydrogen. Rather than fermenting that gas or using thermo-chemical catalysts to produce ethanol, Coskata pumps it into a reactor containing bacteria that consume the gas and excrete ethanol. Richard Tobey, Coskata's vice president of engineering, says the process yields 99.7 percent pure ethanol.

Image Credit: Coskata

Gasification and bacterial conversion are common methods of producing ethanol, but biofuel experts said Coskata is the first to combine them. Doing so, they said, merges the feedstock flexibility of gasification with the relatively low cost of bacterial conversion.
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Coskata's method generates more ethanol per ton of feedstock than corn-based ethanol and requires far less water, heat and pressure. Those cost savings allow it to turn, say, two bales of hay into five gallons of ethanol for less than $1 a gallon, the company said. Corn-based ethanol costs $1.40 a gallon to produce, according to the Renewable Fuels Association.
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May Wu, an environmental scientist at Argonne National Laboratory, says Coskata's ethanol produces 84 percent less greenhouse gas than fossil fuel even after accounting for the energy needed to produce and transport the feedstock. It also generates 7.7 times more energy than is required to produce it. Corn ethanol typically generates 1.3 times more energy than is used producing it.
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Making ethanol is one thing, but there's almost no infrastructure in place for distributing it. But the company's method solves that problem because ethanol could be made locally from whatever feedstock is available, Tobey said.

"You're not bound by location," he said. "If you're in Orange County, you can use municipal waste. If you're in the Pacific Northwest, you can use wood waste. Florida has sugar. The Midwest has corn. Each region has been blessed with the ability to grow its own biomass."
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"Even if you produce it county by county, you still need an infrastructure," he said. "People aren't going to go to some remote location for fuel."
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The Biodesign Institute of Arizona State University - Alternative Engergy
(click image to launch video)

The biofuel developed for ASU's "Tubes in the Desert" project avoids many of the downsides presented by biofuels such as corn, cellulose or other crops/plants. Because it uses a microscopic bacteria as the fuel source, it doesn't compete with food crops and could yield a much larger amount of fuel per acre. The bacteria are grown in transparent tubes, hence the name. /// ASU researchers are also exploring the possibilities of microbial fuel cells -- tiny microbes that generate energy by feeding on waste. /// Guest interviews include: Neal Woodbury, Ph.D., the Biodesign Institute; Wim Vermaas, Professor, ASU School of Life Sciences. /// Learn More: Visit http://www.azpbs.org/asuspotlight

Microbe Petrol For Better Living

Better biofuel: Stephen del Cardayre, a biochemist and LS9's vice president for research and development. Image Credit: Saul Bromberger and Sandra Hoover

Microbe Petrol For Better Living

What would happen if technology could deliver a renewable way of creating gasoline in relative minutes as opposed to the millions and millions of years it takes to create the base of gasoline we pump out of the ground (you know, the type that comes from dinosaur remains?

Well, if companies like LS9 and Amyris Biotechnologies have their way, we all will be pumping gas into our tanks that is created through having bacteria make hydrocarbons.

If this technology conversion can be made viable, it would lend a new interpretation to the expression – “Man, that car is SICK!”

This excerpted from Massachusetts Institute of Technology’s Technology Review -

Making Gasoline from Bacteria
A biotech startup wants to coax fuels from engineered microbes.
By Neil Savage, Technology Review - Wednesday, August 01, 2007

The biofuel of the future could well be gasoline. That's the hope of one biotech startup that on Monday described for the first time how it is coaxing bacteria into producing hydrocarbons that could be processed into fuels like those made from petroleum.

LS9, a company based in San Carlos, CA, and founded by geneticist George Church, of Harvard Medical School, and plant biologist Chris Somerville, of Stanford University, had previously said that it was working on what it calls "renewable petroleum." But at a Society for Industrial Microbiology conference on Monday, the company began speaking more openly about what it has accomplished: it has genetically engineered various bacteria, including E. coli, to custom-produce hydrocarbon chains.

E. coli - Image Credit: uni-heidelberg.de

To do this, the company is employing tools from the field of synthetic biology to modify the genetic pathways that bacteria, plants, and animals use to make fatty acids, one of the main ways that organisms store energy. Fatty acids are chains of carbon and hydrogen atoms strung together in a particular arrangement, with a carboxylic acid group made of carbon, hydrogen, and oxygen attached at one end. Take away the acid, and you're left with a hydrocarbon that can be made into fuel.

"I am very impressed with what they're doing," says James Collins, codirector of the Center for Advanced Biotechnology at Boston University. He calls the company's use of synthetic biology and systems biology to engineer hydrocarbon-producing bacteria "cutting edge."

In some cases, LS9's researchers used standard recombinant DNA techniques to insert genes into the microbes. In other cases, they redesigned known genes with a computer and synthesized them. The resulting modified bacteria make and excrete hydrocarbon molecules that are the length and molecular structure the company desires.

Stephen del Cardayre, a biochemist and LS9's vice president for research and development, says the company can make hundreds of different hydrocarbon molecules. The process can yield crude oil without the contaminating sulfur that much petroleum out of the ground contains. The crude, in turn, would go to a standard refinery to be processed into automotive fuel, jet fuel, diesel fuel, or any other petroleum product that someone wanted to make.

Next year LS9 will build a pilot plant in California to test and perfect the process, and the company hopes to be selling improved biodiesel and providing synthetic biocrudes to refineries for further processing within three to five years. (See "Building Better Biofuels.")

But LS9 isn't the only company in this game. Amyris Biotechnologies, of Emeryville, CA, is also using genes from plants and animals to make microbes produce designer fuels. Neil Renninger, senior vice president of development and one of the company's cofounders, says that Amyris has also created bacteria capable of supplying renewable hydrocarbon-based fuels. The main difference between the companies, Renninger says, is that while LS9 is working on a biocrude that would be processed in a refinery, Amyris is working on directly producing fuels that would need little or no further processing.
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LS9's current work uses sugar derived from corn kernels as the food source for the bacteria--the same source used by ethanol-producing yeast. To produce greater volumes of fuel, and to not have energy competing with food, both approaches will need to use cellulosic biomass, such as switchgrass, as the feedstock. Del Cardayre estimates that cellulosic biomass could produce about 2,000 gallons of renewable petroleum per acre.

Producing hydrocarbon fuels is more efficient than producing ethanol, del Cardayre adds, because the former packs about 30 percent more energy per gallon. And it takes less energy to produce, too. The ethanol produced by yeast needs to be distilled to remove the water, so ethanol production requires 65 percent more energy than hydrocarbon production does.

The U.S. Department of Energy has set a goal of replacing 30 percent of current petroleum use with fuels from renewable biological sources by 2030, and del Cardayre says he feels that's easily achievable.

Reference Here>>